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Biswas, S. K.
- Characterization of Antifungal Metabolites of Chaetomium globosum Kunze and their Antagonism against Fungal Plant Pathogens
Authors
1 Department of Plant Pathology, C.S.A. University of Agriculture and Technology, Kanpur 208002, IN
2 Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi 110012, IN
3 Division of Agricultural Chemicals, Indian Agricultural Research Institute, New Delhi 110012, IN
Source
Journal of Biological Control, Vol 26, No 1 (2012), Pagination: 70-74Abstract
Chaetomium species which are normally found in soil and organic compost are noted for the presence of secondary metabolite with biological activities. Secondary metabolites from culture filtrate of Chaetomium globosum Kunze were extracted by solvent extraction method using ethyl acetate and separated by thin layer chromatography in five major bands of different Rf values. These compounds were further purified and fractioned with column chromatography. Compound '1' eluted with hexane (fraction 1-18) as color less liquid (Rf 0.84), and other five compounds (2,3,4,5,6) with different ratio of hexane, benzene and acetone as solvent in different fractions as: 34-63 (Rf. 0.46), 71-75 (Rf 0.31), 78 (Rf 0.58), 76-77 (Rf 0.58) and 85-89 (Rf 0.47) were eluted and purified. GS-MS and NMR studies revealed that compounds 2-6 were identical to spectral data of metabolites, chaetomin, BHT, mollicelin G, isomer of mollicelin G and cochiliodinol respectively. One more elution with benzene: acetone (95 : 5 v/v) gave a pale yellow crystalline compound of Rf 0.37 identified as chaetoglobosin. Bioassay studies with two compounds i.e., chaetoglobosin and chaetomin revealed significant growth inhibitory activity against various plant pathogens such as Bipolaris sorokiniana, Macrophomina phaseolina, Rhizoctonia solani and Pythium ultimum under in vitro conditions.Keywords
Chaetomium globosum, Biocontrol and Secondary Metabolites, HPLC GC- MS.References
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- Integrated disease management approaches for control of late blight of potato and enhancing the growth of potato
Authors
1 Department of Plant Pathology, C. S. Azad University of Agriculture and Tech, Kanpur – 200802, Uttar Pradesh, IN
Source
Journal of Biological Control, Vol 32, No 4 (2018), Pagination: 264-269Abstract
Experiments were conducted to study integrated approach in the management of potato blight. The result showed that T1 treatment (soil application of FYM @ 125gm/pot + waste of mushroom cultivation with Trichoderma harzianum @ 5gm/kg + tuber treatment with Azotobacter @ 5% + foliar spray with Ridomil @ 0.25%) was the best to show minimum disease severity, representing 9.16% as against 76.40% in case of control at 21 days of observation. The treatment also exhibited increased tuber germination and plant height of potato showing the value 100 per cent and 44.5 cm at 30 days age of plant. The maximum number of large size tuber and total yield with 668.6g was also recorded from treatment T1. Thus, integration of FYM + mushroom waste as soil application + bio formulation as tuber treatment + fungicides as foliar spray can be efficiently applied for management of late blight and also get enhanced yield of potato.
Keywords
Bioagent, Disease Severity, Growth Parameter, Late Blight, Organic Matter, Potato.References
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